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Image Search Results
Journal: Nature Communications
Article Title: Long non-coding RNA-dependent mechanism to regulate heme biosynthesis and erythrocyte development
doi: 10.1038/s41467-018-06883-x
Figure Lengend Snippet: UCA1 depletion impairs heme metabolism and blocks erythroid maturation. a The relative UCA1 expression was quantitated by qRT-PCR on days 8 and 14 in shRNA or control lentivirus (EV) infected primary erythroid cells. 18S rRNA was used as the internal control. b – h Human primary erythroid differentiation was monitored by flow cytometry (CD71 + CD235a + cells) ( b , c ), Wright-Giemsa staining ( d , Scale bar = 10 μm), the cellular ( e ) and nuclear size ( f ) (on day 14, n = 100), and benzidine staining ( g , h , Scale bar = 20 μm) on days 14 in shRNA lentivirus infected cells or control lentivirus (EV) infected cells. i The heatmap depicts DEGs profiling after UCA1 depletion (shRNA#2) in differentiated erythroblasts at day 8 ( P adj < 0.05 and FPKM > 0.1). j Hallmark gene set enrichment analysis of DEGs after UCA1 knockdown. Bar graphs were generated with data from three independent experiments ( n = 3). Error bars represent SEM. P -values were determined by Student’s t -test. *P < 0.05, **P < 0.01
Article Snippet: GSEA was conducted, and the plots was generated by
Techniques: Expressing, Quantitative RT-PCR, shRNA, Control, Infection, Flow Cytometry, Staining, Knockdown, Generated
Journal: Nature Communications
Article Title: Long non-coding RNA-dependent mechanism to regulate heme biosynthesis and erythrocyte development
doi: 10.1038/s41467-018-06883-x
Figure Lengend Snippet: Linking UCA1 and PTBP1-regulated erythroid maturation to the control of heme biosynthesis. a The heatmap depicts the fold-change in DEGs after UCA1 (shRNA#2/EV) or PTBP1 depletion (shRNA#1/EV) in differentiated erythroblasts at day 8. b Hallmark enrichment analysis of the common DEGs. c GSEA showed heme metabolism gene set enrichment from the common DEGs after UCA1 (left) or PTBP1 (right) depletion. Normalized enrichment scores (NES) and P -values are indicated in each plot. d Heatmap shows the expression of heme metabolism related genes after UCA1 or PTBP1 downregulation. e GO analysis of the common heme metabolism related genes after UCA1 or PTBP1 depletion. f , g qRT-PCR to analyze the expression of genes involved in heme biosynthesis on days 8 in differentiated primary erythroid cells after UCA1 ( f ) or PTBP1 ( g ) depletion. GAPDH mRNA was used as an internal control. Bar graphs were generated with data from three independent experiments ( n = 3). Error bars represent SEM. P -values were determined by Student’s t -test. *P < 0.05, **P < 0.01
Article Snippet: GSEA was conducted, and the plots was generated by
Techniques: Control, shRNA, Expressing, Quantitative RT-PCR, Generated